Replaces accession AW330728
putative gag-pol precursor
Annotation based upon blastx and blastn hits.
BLastX:
gi|33113963|gb|AAP94585.1| putative gag-pol precursor [Zea mays] 298 1e-79
gi|37533914|ref|NP_921259.1| putative retroelement [Oryza sat... 185 1e-45
gi|50930635|ref|XP_474845.1| OSJNBa0035O13.3 [Oryza sativa (j... 185 1e-45
gi|77555623|gb|ABA98419.1| retrotransposon protein, putative,... 183 5e-45
gi|77554754|gb|ABA97550.1| retrotransposon protein, putative,... 182 6e-45
gi|32488627|emb|CAE03420.1| OSJNBa0032F06.3 [Oryza sativa (ja... 180 4e-44
gi|90399003|emb|CAJ86273.1| H0901F07.10 [Oryza sativa (indica cu 174 2e-42
gi|77551287|gb|ABA94084.1| retrotransposon protein, putative,... 174 2e-42
gi|77552111|gb|ABA94908.1| retrotransposon protein, putative,... 174 3e-42
Top Hit:
>gi|33113963|gb|AAP94585.1| putative gag-pol precursor [Zea mays]
Length=1833
Score = 298 bits (763), Expect = 1e-79
Identities = 155/205 (75%), Positives = 159/205 (77%), Gaps = 42/205 (20%)
Frame = +1
Query 7 QFMAIPHYTYMILKMPGPQGIITVRADFQGAAECFRVAIQAALTTKPSTVPSTPANSKPE 186
+FMAIPHYTYMILKMPGP+GIITVRADFQGAAECFRVAIQAALTTKPS VP+T ANSKPE
Sbjct 788 KFMAIPHYTYMILKMPGPRGIITVRADFQGAAECFRVAIQAALTTKPSAVPATSANSKPE 847
Query 187 EDLAVPANEAQAVTSMRPTEETKRINLGFADERKTAIISSSLNDK*ENALVQFLQDNRDV 366
EDL E+ALVQFLQDNRDV
Sbjct 848 EDLT------------------------------------------ESALVQFLQDNRDV 865
Query 367 FTWQPADMPGVPRELAEHKLKVYPQARPIRQKLRRFTPDKREAIRAELARLVAAGFIREV 546
F WQPADMPGVPRELAEHKLKVYPQARPIRQKLRRFTPDKREAIRAELARLVAAGFIREV
Sbjct 866 FAWQPADMPGVPRELAEHKLKVYPQARPIRQKLRRFTPDKREAIRAELARLVAAGFIREV 925
Query 547 LHPEWLANPVLVLKKNKVDWRMCVD 621
LHPEWLANPVLVLKKNKVDWRMCVD
Sbjct 926 LHPEWLANPVLVLKKNKVDWRMCVD 950
Repeats
>DV550068 1000059-A04.GAD10-F UGI-Reseq Zea mays cDNA, mRNA sequence. (627 bp masked -- 100.00% masked) GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
ZRSiTERTOOT0161 grande_238E11-1 grande_rp1 ||retrotransposon 991 0.0
ZRSiTERTOOT0159 grande_013I05-1 grande_Z013I05-1 Z013I05 bases 3... 880 0.0
ZRSiTERTOOT0001 gi|1617472|emb|X97605.1|ZDGRAND16 Z.diploperenni... 811 0.0
ZRSiTERTOOT0166 grande_af546188-1 grande_af546188-1 af546188 bas... 795 0.0
GRANDE1_ZD#LTR/Zea Z.diploperennis DNA for Grande1-4 retrotransp... 781 0.0
ZRSiTERTOOT0165 grande_af448416-1 grande_af448416-1 af448416 bas... 733 0.0
ZRSiTERTOOT0162 grande_464F01-1part grande_Z464F01-1part ZZ464F0... 729 0.0
ZRSiTERTOOT0163 grande_573F08-1hdnm grande_Z573F08-1hdnm grande_... 640 0.0
Interproscan:
no hits reported.
Related Literature:
J Virol. 2006 Nov;80(21):10478-86.
Links
Human immunodeficiency virus type 1 Gag polyprotein modulates its own translation.
Department of Medicine, University of Cambridge, Level 5, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, United Kingdom.
The full-length viral RNA of human immunodeficiency virus type 1 (HIV-1) functions both as the mRNA for the viral structural proteins Gag and Gag/Pol and as the genomic RNA packaged within viral particles. The packaging signal which Gag recognizes to initiate genome encapsidation is in the 5' untranslated region (UTR) of the HIV-1 RNA, which is also the location of translation initiation complex formation. Hence, it is likely that there is competition between the translation and packaging processes. We studied the ability of Gag to regulate translation of its own mRNA. Gag had a bimodal effect on translation from the HIV-1 5' UTR, stimulating translation at low concentrations and inhibiting translation at high concentrations in vitro and in vivo. The inhibition was dependent upon the ability of Gag to bind the packaging signal through its nucleocapsid domain. The stimulatory activity was shown to depend on the matrix domain of Gag. These results suggest that Gag controls the equilibrium between translation and packaging, ensuring production of enough molecules of Gag to make viral particles before encapsidating its genome.
PMID: 17041220 [PubMed - indexed for MEDLINE]
